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Performance Evaluation of the AcT 5diff Hematology Analyzer

Performance Evaluation of the Complete Blood Count and White Blood Cell Differential Parameters on the AcT 5diff Hematology Analyzer

Patrick O'Neil, Esther Vital, Noemi Betancourt-Loria, Dinah Montes
Beckman Coulter, Inc, Diagnostics Division, Miami, Florida

Laboratory Hematology 7:116-124
© 2001 Carden Jennings Publishing Co., Ltd.

The Coulter AcT 5diff Hematology Analyzer from Beckman Coulter (Miami, FL) is a benchtop system designed for the low-volume laboratory. Its small sample size requirement, capability for complete blood count (CBC) with 5-part differential, and convenient reagents make it ideal for use in this environment. The system was evaluated to compare its CBC and 5-part white blood cell (WBC) differential performance with that of the midvolume Coulter HmX analyzer. The clinical utility and flagging performance of the automated analyzers were confirmed by 100-cell manual differential counts. The AcT 5diff analyzer was shown to have excellent precision and accuracy for the CBC parameters, with coefficients of correlation >0.95 for directly measured parameters. The WBC differential results from the AcT 5diff system compared well to other automated differential results, and flagged morphologically abnormal samples were confirmed as appropriate by the manual differential. An efficiency rate >80% means the laboratory will properly identify abnormal specimens for follow-up but will not be burdened with unnecessary manual differentials.

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sm_cjpLogo.gifCopyright 1995-2010 - Carden Jennings Publishing Co., Ltd. All rights reserved. The material available at this site is for educational purposes only and is NOT intended for any diagnostic, clinically related, or other purpose. Carden Jennings Publishing Co., Ltd., assumes no responsibility for any use or misuse of this material and makes no warranty or representation of any kind with respect to the material available at this site.

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