Species-Specific FcR Blocking
Species-Specific Blocking of Fc Receptors in Indirect Immunofluorescence Assays
Peter Sedlmayr, Verena Leitner, Stefan Pilz, Reinhold Wintersteiger, Carleton C. Stewart, Gottfried Dohr
Laboratory Hematology 7:81-84
©2001 Carden Jennings Publishing Co., Ltd.
Fc receptor (FcR) binding of antibodies to the surface of cells is a cause of background fluorescence and reduces the sensitivity of specific epitope-binding immunofluorescence. Pretreatment of cells with immunoglobulin can reduce background staining due to FcRs. We compared the capacity of goat immunoglobulin (Ig)G and human IgG to block Fc binding to human monocytic cells stimulated with interferon-y and granulocyte-macrophage colony-stimulating factor. We found that the choice of the species of the immunoglobulin is relevant for the efficiency of blocking. Goat IgG is not an effective FcR block, whereas human IgG blocks human Fc receptors efficiently.
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